Validation of ToLCNDV resistant germplasm in melon and cucumber through challenged inoculation

Authors

  • Pragya Ranjan ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110 012, India
  • Amlendu Ghosh ICAR-Indian Agricultural Research Institute, Pusa, New Delhi 110 012, India
  • Shiksha Bhandari ICAR-Indian Agricultural Research Institute, New Delhi 110012 India
  • J K Ranjan ICAR-Indian Agricultural Research Institute, Pusa, New Delhi 110 012, India
  • Pavan Kumar Malav ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110 012, India
  • Anjula Pandey ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110 012, India
  • G P SIngh ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110 012, India

Keywords:

ToLCNDV, resistant germplasm, cucumber, melon, vulnerability index, artificial screening, challenged inoculation

Abstract

The present study was conducted to validate the Tomato Leaf Curl New Delhi Virus (ToLCNDV) resistance in four promising accessions of melon and cucumber (IC629818-A, IC629818-B, IC629822, and IC410617) along with checks PI 124111, Pusa Uday and Punjab Naveen through artificial inoculation at glasshouse facility of ICAR-IARI, New Delhi during 2024-25. These lines were identified through field screening against ToLCNDV in the Kharif season at ICAR-NBPGR farm during 2021-2024. The artificially inoculated glasshouse grown plants of Arya IC629818-A and IC629818-B showed no symptoms of ToLCNDV with 0 velnerability index. PCR amplification also confirmed the absence of virus in the plants. This is perhaps the first report wherein any melon germplasm showed immune/highly resistant reaction to ToLCNDV. The cucumber genotype IC410617 had little symptom (VI=4.5) with no amplification of viral coat protein and thus categorized as resistant.  These lines show potential for their utilization as novel sources of resistance for breeding of ToLCNDV-resistant cucumber and melon cultivars.

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Published

2026-03-24

How to Cite

Ranjan, P. ., Ghosh, A. ., Bhandari, S., Ranjan, J. K., Malav, P. K., Pandey, A., & Singh, G. P. (2026). Validation of ToLCNDV resistant germplasm in melon and cucumber through challenged inoculation. Current Horticulture, 14(1), 85–87. Retrieved from https://www.currenthorticulture.com/index.php/CURHOR/article/view/274

Issue

Vol. 14 No. 1 (2026): January-March 2026

Section

Short-Communication

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